Real SVF Cell Isolation
Cellanova SVF Kit offers a system designed to obtain high mesenchymal stem cell yields without the use of enzymes while providing a significantly faster workflow and substantially lower investment costs compared to enzymatic systems. Discover the future of regenerative therapies with D-MED.
Why Cellanova SVF
We do not define the process of making adipose tissue injectable as stem cell therapy. SVF cells are genuinely isolated from the extracellular matrix/tissue for you.
While the final product is rich in nucleated cells, fibrotic tissue, adipocytes, and triglycerides are effectively excluded. In addition, 41 μm filtration applied to the final product helps provide a safer injectable cellular suspension.
Fat injection primarily provides a mechanical volumizing effect due to its structural properties. However, it cannot deliver the same high regenerative potential as concentrated SVF isolation enriched with stem cells.
Stem cells isolated and concentrated from tissue can rapidly proliferate within the body’s natural microenvironment and differentiate into various cell types. Unlike standard fat injection procedures, this approach offers a true regenerative treatment opportunity.
Micronized adipose tissues may contain stem cells; however, this does not mean that SVF cell populations are isolated, purified, or concentrated.
With Cellanova SVF, the final product can be directly visualized under a standard microscope. Furthermore, under fluorescence microscopy, nucleated and non-nucleated cells can be identified, quantified, and their viability can be easily evaluated.
Measured in the lab
Immediately After SVF Cell Isolation
Fluorescence Microscopy Count and Analysis Results
Total cell (x 10⁷ cells/mL)
Nucleated cell (x 10⁶ cells/mL)
Non-nucleated cell (x 10⁷ cells/mL)
Viability of nucleated cell
Data obtained from samples dated 28 December 2020. Cell counting was performed using the LunaSTEM system, and the report was provided by Seher Üstün Yaylacı, MD, Assoc. Prof.
Flow cytometry
FITC: CD73
APC: CD90
PerCP: CD105
Flow cytometry analysis was performed to distinguish hematopoietic and endothelial cell populations. Surface antigens including CD34, CD29, CD14, CD11b, and HLA-DR were evaluated using a lineage exclusion strategy, enabling the determination of mesenchymal, endothelial, pericytic, leukocytic, and stromal/progenitor cell fractions in the final product.
The study was conducted on samples dated 28 December 2020 by Maia Health Consultancy at Hacettepe University Teknokent. Cell count results were derived from the report of by Seher Üstün Yaylacı, MD, Assoc. Prof. All analyses were performed using the BD FACSCanto II device and BD FACS Diva software.
Scientific publications
Michele L. Zocchi, MD, PhD, Prof.
“Many years have passed since my publication in 1988, which was the first Adipose Tissue Derived SVF study to enter the medical literature with a proprietary manual centrifugation system that I developed and patented. Over the years, I have developed, tested and mastered many different techniques that offer both mechanical and enzymatic SVF approaches. When evaluating a new medical device for concentrating, preparing and isolating SVF, I have always considered ease of use, speed, efficiency and cost. Mechanical SVF technology, which I covered extensively in my Masterclass published in the European Journal of Plastic Surgery (EJPS) in 2019, has achieved the most satisfactory results in all these criteria beyond other devices I have experienced.”
Alper Murat Ulaşlı, MD, Prof.
“Adipose tissue-derived stromal cells (ADSCs) play a crucial role in research and applications of regenerative medicine as they can be rapidly isolated in high quantities. However, their purity, pluripotent properties, differentiation capacity, stem cell marker expression can vary greatly depending on the technique and the means used for extraction. In my study published in Nature Scientific Reports in 2023, I investigated the performance of the widely used Mechanical SVF technology in separating Adipose-derived Stem Cells and its advantages over the enzyme method, which is considered the golden method, as well as cell quality.”
Pietro Gentile, MD, Assoc. Prof.
“This study aimed to evaluate the long-term effects of the widely used fat grafting enhanced with adipose tissue-derived mesenchymal stem cells to treat scars with pigmentary changes and soft tissue deformities. Published in 2023 in Aesthetic Plastic Surgery, I described the efficacy and long-term regeneration of fat injections in scar treatments.”
Michele L. Zocchi, MD, PhD, Prof.
“We analyzed the main causes of cell death and strategies to prevent it. We present you all the technical steps to achieve the most efficient regenerative effect in different clinical and pathological conditions and to prepare different mixing methods with its main ingredients. In 2021, in my Masterclass published in the European Journal of Plastic Surgery (EJPS), I shared my results in different disciplines with new techniques.”
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